Osteogenesis response to implanted materials in endocortical bone in rat femora.
Identifieur interne : 000279 ( Main/Exploration ); précédent : 000278; suivant : 000280Osteogenesis response to implanted materials in endocortical bone in rat femora.
Auteurs : Xudong Li [République populaire de Chine] ; Ge Feng ; Songsong Zhu ; Jihua Li ; En Luo ; Jing HuSource :
- Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association [ 1436-2023 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- anatomie et histologie : Fémur.
- physiologie : Fémur, Ostéogenèse.
- Animaux, Biosynthèse des protéines, Densité osseuse, Expression des gènes, Phénomènes biomécaniques, Rat Sprague-Dawley, Rats.
English descriptors
- KwdEn :
- MESH :
- anatomy & histology : Femur.
- physiology : Femur, Osteogenesis.
- Animals, Biomechanical Phenomena, Bone Density, Gene Expression, Protein Biosynthesis, Rats, Rats, Sprague-Dawley.
Abstract
BACKGROUND
Orthopedic surgeons and dentists often implant materials to repair bone tissue defects and restore physiological functions of bone organs. The clinical success depends on adequate bone formation in operation sites. However, the real cause of osteogenesis has not yet been fully elucidated. To investigate the bone response to implanted materials, this study examined the bone tissue reaction in rat femoral medullary canal, which received gelatin and collagen as foreign-body materials.
METHODS
A total of 36 six-month-old Sprague-Dawley rats were randomly and meanly divided into three groups. In the gelatin group, the bilateral femora received gelatin material; in the collagen group, they were implanted with type I collagen, and in the control group, the femora suffered from sham operation with no materials inserted. After 2, 4, 8, and 12 weeks, specimens were harvested and subjected to a series of examinations.
RESULTS
After 2 weeks of healing, a significant upregulation of both alkaline phosphatase and osteocalcin by both kinds of implanted materials relative to the control (sham implantation group) was seen in gene expression analysis. Strong reactivity of osteoprotegerin and receptor activator of NFκB ligand was detected in the two test groups in immunohistochemistry at 4 weeks of healing. Also, micro-CT revealed an increase in cortical bone thickness in the two test groups as compared to the control group. Densitometry showed increased bone mineral density in the bone receiving materials after 12 weeks, leading to the enhanced maximum load in the test groups.
CONCLUSIONS
These results indicated that the implanted materials led to an osteogenesis response in rat femoral medullary canal. Thus, we probably should reconsider the potential cascades of tissue reaction when utilizing orthopedic and dental implants and other materials to recover bone related-organ function and repair bone defects.
DOI: 10.1007/s00776-012-0254-4
PubMed: 22739934
Affiliations:
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last="Zhu">Songsong Zhu</name></author><author><name sortKey="Li, Jihua" sort="Li, Jihua" uniqKey="Li J" first="Jihua" last="Li">Jihua Li</name></author><author><name sortKey="Luo, En" sort="Luo, En" uniqKey="Luo E" first="En" last="Luo">En Luo</name></author><author><name sortKey="Hu, Jing" sort="Hu, Jing" uniqKey="Hu J" first="Jing" last="Hu">Jing Hu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22739934</idno><idno type="pmid">22739934</idno><idno type="doi">10.1007/s00776-012-0254-4</idno><idno type="wicri:Area/Main/Corpus">000277</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000277</idno><idno type="wicri:Area/Main/Curation">000277</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000277</idno><idno type="wicri:Area/Main/Exploration">000277</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Osteogenesis response to implanted materials in endocortical bone in rat femora.</title><author><name sortKey="Li, Xudong" sort="Li, Xudong" uniqKey="Li X" first="Xudong" last="Li">Xudong Li</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu</wicri:regionArea><wicri:noRegion>Chengdu</wicri:noRegion></affiliation></author><author><name sortKey="Feng, Ge" sort="Feng, Ge" uniqKey="Feng G" first="Ge" last="Feng">Ge Feng</name></author><author><name sortKey="Zhu, Songsong" sort="Zhu, Songsong" uniqKey="Zhu S" first="Songsong" last="Zhu">Songsong Zhu</name></author><author><name sortKey="Li, Jihua" sort="Li, Jihua" uniqKey="Li J" first="Jihua" last="Li">Jihua Li</name></author><author><name sortKey="Luo, En" sort="Luo, En" uniqKey="Luo E" first="En" last="Luo">En Luo</name></author><author><name sortKey="Hu, Jing" sort="Hu, Jing" uniqKey="Hu J" first="Jing" last="Hu">Jing Hu</name></author></analytic><series><title level="j">Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association</title><idno type="eISSN">1436-2023</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Biomechanical Phenomena (MeSH)</term><term>Bone Density (MeSH)</term><term>Femur (anatomy & histology)</term><term>Femur (physiology)</term><term>Gene Expression (MeSH)</term><term>Osteogenesis (physiology)</term><term>Protein Biosynthesis (MeSH)</term><term>Rats (MeSH)</term><term>Rats, Sprague-Dawley (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Biosynthèse des protéines (MeSH)</term><term>Densité osseuse (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Fémur (anatomie et histologie)</term><term>Fémur (physiologie)</term><term>Ostéogenèse (physiologie)</term><term>Phénomènes biomécaniques (MeSH)</term><term>Rat Sprague-Dawley (MeSH)</term><term>Rats (MeSH)</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Fémur</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Femur</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fémur</term><term>Ostéogenèse</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Femur</term><term>Osteogenesis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biomechanical Phenomena</term><term>Bone Density</term><term>Gene Expression</term><term>Protein Biosynthesis</term><term>Rats</term><term>Rats, Sprague-Dawley</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biosynthèse des protéines</term><term>Densité osseuse</term><term>Expression des gènes</term><term>Phénomènes biomécaniques</term><term>Rat Sprague-Dawley</term><term>Rats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Orthopedic surgeons and dentists often implant materials to repair bone tissue defects and restore physiological functions of bone organs. The clinical success depends on adequate bone formation in operation sites. However, the real cause of osteogenesis has not yet been fully elucidated. To investigate the bone response to implanted materials, this study examined the bone tissue reaction in rat femoral medullary canal, which received gelatin and collagen as foreign-body materials.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>A total of 36 six-month-old Sprague-Dawley rats were randomly and meanly divided into three groups. In the gelatin group, the bilateral femora received gelatin material; in the collagen group, they were implanted with type I collagen, and in the control group, the femora suffered from sham operation with no materials inserted. After 2, 4, 8, and 12 weeks, specimens were harvested and subjected to a series of examinations.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>After 2 weeks of healing, a significant upregulation of both alkaline phosphatase and osteocalcin by both kinds of implanted materials relative to the control (sham implantation group) was seen in gene expression analysis. Strong reactivity of osteoprotegerin and receptor activator of NFκB ligand was detected in the two test groups in immunohistochemistry at 4 weeks of healing. Also, micro-CT revealed an increase in cortical bone thickness in the two test groups as compared to the control group. Densitometry showed increased bone mineral density in the bone receiving materials after 12 weeks, leading to the enhanced maximum load in the test groups.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>These results indicated that the implanted materials led to an osteogenesis response in rat femoral medullary canal. Thus, we probably should reconsider the potential cascades of tissue reaction when utilizing orthopedic and dental implants and other materials to recover bone related-organ function and repair bone defects.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22739934</PMID><DateCompleted><Year>2013</Year><Month>04</Month><Day>03</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1436-2023</ISSN><JournalIssue CitedMedium="Internet"><Volume>17</Volume><Issue>5</Issue><PubDate><Year>2012</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association</Title><ISOAbbreviation>J Orthop Sci</ISOAbbreviation></Journal><ArticleTitle>Osteogenesis response to implanted materials in endocortical bone in rat femora.</ArticleTitle><Pagination><MedlinePgn>626-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00776-012-0254-4</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Orthopedic surgeons and dentists often implant materials to repair bone tissue defects and restore physiological functions of bone organs. The clinical success depends on adequate bone formation in operation sites. However, the real cause of osteogenesis has not yet been fully elucidated. To investigate the bone response to implanted materials, this study examined the bone tissue reaction in rat femoral medullary canal, which received gelatin and collagen as foreign-body materials.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">A total of 36 six-month-old Sprague-Dawley rats were randomly and meanly divided into three groups. In the gelatin group, the bilateral femora received gelatin material; in the collagen group, they were implanted with type I collagen, and in the control group, the femora suffered from sham operation with no materials inserted. After 2, 4, 8, and 12 weeks, specimens were harvested and subjected to a series of examinations.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">After 2 weeks of healing, a significant upregulation of both alkaline phosphatase and osteocalcin by both kinds of implanted materials relative to the control (sham implantation group) was seen in gene expression analysis. Strong reactivity of osteoprotegerin and receptor activator of NFκB ligand was detected in the two test groups in immunohistochemistry at 4 weeks of healing. Also, micro-CT revealed an increase in cortical bone thickness in the two test groups as compared to the control group. Densitometry showed increased bone mineral density in the bone receiving materials after 12 weeks, leading to the enhanced maximum load in the test groups.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">These results indicated that the implanted materials led to an osteogenesis response in rat femoral medullary canal. Thus, we probably should reconsider the potential cascades of tissue reaction when utilizing orthopedic and dental implants and other materials to recover bone related-organ function and repair bone defects.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xudong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Ge</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Songsong</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jihua</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>En</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Jing</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>06</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Orthop Sci</MedlineTA><NlmUniqueID>9604934</NlmUniqueID><ISSNLinking>0949-2658</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001696" MajorTopicYN="N">Biomechanical Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015519" MajorTopicYN="N">Bone Density</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005269" MajorTopicYN="N">Femur</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010012" MajorTopicYN="N">Osteogenesis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014176" MajorTopicYN="N">Protein Biosynthesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017207" MajorTopicYN="N">Rats, Sprague-Dawley</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>12</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>05</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>6</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>6</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>4</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22739934</ArticleId><ArticleId IdType="doi">10.1007/s00776-012-0254-4</ArticleId><ArticleId IdType="pii">S0949-2658(15)30612-6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Feng, Ge" sort="Feng, Ge" uniqKey="Feng G" first="Ge" last="Feng">Ge Feng</name><name sortKey="Hu, Jing" sort="Hu, Jing" uniqKey="Hu J" first="Jing" last="Hu">Jing Hu</name><name sortKey="Li, Jihua" sort="Li, Jihua" uniqKey="Li J" first="Jihua" last="Li">Jihua Li</name><name sortKey="Luo, En" sort="Luo, En" uniqKey="Luo E" first="En" last="Luo">En Luo</name><name sortKey="Zhu, Songsong" sort="Zhu, Songsong" uniqKey="Zhu S" first="Songsong" last="Zhu">Songsong Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Li, Xudong" sort="Li, Xudong" uniqKey="Li X" first="Xudong" last="Li">Xudong Li</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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